NBS-C  BioScience 
Monoclonal Antibodies; Recombinant Proteins

Anti-human IgE monoclonal antibody (BSW17)

This mouse monoclonal antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signalling and to detect free (non receptor bound) IgE in human serum samples.

Anti-human IgE monoclonal antibody (Le27)

This mouse monoclonal antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound and total IgE in human serum samples. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.

Anti-human high affinity IgE receptor (FceRIa) antibody (IM-2)

This mouse monoclonal antibody recognizes an epitope within the a2 domain of the human high affinity IgE receptor (FceRIa). It inhibits binding of IgE to this receptor and is suitable to detect receptors expressed on primary cells and cell lines (e.g mast cells and basophils). It can also be used as weak to moderate cross-linking agent for IgE-independent triggering of effector cells and to detect FceRIa in ELISA and W-blots

Anti-human high affinity IgE receptor (FceRIa) antibody (SR-1)

This mouse monoclonal antibody recognizes the carboxy-terminal extracellular region of the a2 domain of the human high affinity IgE receptor (FceRIa). It does not inhibit binding of IgE to this receptor and is suitable to detect soluble receptors present in human serum or other research samples. It can be used to detect FceRIa in ELISA and W-blots. 

Recombinant human high affinity IgE receptor, a-chain (FceRIa)

Recombinant protein expressed in CHO cells. Specifically binds to human IgE with an affinity comparable to the native IgE receptor expressed on mast cells and basophils (KD < 1nM). Among other applications it can be used as functional reagent in allergy research, mast cell / basophil biology and as analytical tool for detection and quantification of biologically active (= receptor binding) IgE in experimental cell supernatants and body fluids.