NBS-C  BioScience 
Human Immunoglobulin E (hIgE)

  • Purified by immunoaffinity chromatography on IgE specific monoclonal antibody. High purity confirmed by analytical gel permeation chromatography (Superose 12/HR)
  • Functionally tested by monitoring real time binding kinetics to human high affinity IgE receptor (Surface Plasmon Resonance technology; Biacore™)
  • Available as reference standard, calibrated against the international WHO IgE reference (WHO, 2nd IRP, 75/502) for quantitative IgE measurements with excellent comparability to external (clinical) research data
  • Available in different formulations, adaptable to user’s demand upon request, e.g. buffers, preservatives, labels, bulk orders
  • Used worlwide by researchers in many laboratories since more than 20 years, cited in high class peer reviewed journals

Human IgE protein for allergy research and isotype control

Recombinant human IgE (clone SUS-11-NBS01) produced from a human B cell hybridoma cell line established from a healthy donor (CD40 cell culture system). IgE (SUS-11) binds to the alpha-chain of the human high affinity IgE receptor and the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used for IgE monitoring in biological research samples (ELISA; FACS; W-blot, isotype control) or as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs like NBS-C BioScience mAb LE27 or a specific recombinant cross-linking agent.

Human IgE reference protein for calibrated ELISA development

Monoclonal human IgE protein to be used as reference for quantitative IgE concentration measurements in human blood serum, biological research samples, cell culture supernatants, body fluids or tissue extracts. The IgE reference standard is calibrated against the international WHO IgE reference (WHO, 2nd IRP, 75/502). It is intended to be used by researchers engaged in biological and clinical R&D in allergology and any other research field connected to investigation of IgE dependent biological systems, in particular as calibrated reference standard for human serum IgE ELISA.