NBS-C  BioScience 
IgEQUANT® - Human IgE Detection Tools

IgEQUANT®-G 
  

General purpose sandwich ELISA assay tool kit for quantitative determination of total human IgE in biological and clinical research samples, not for use in clinical diagnostic procedures. Protocol optimized for sample concentrations between 20ng and 12µg IgE/ml (~ 10 and 5000 IU/ml).

IgEQUANT®- S

Sensitive sandwich ELISA assay tool kit for quantitative determination of total human IgE in biological research samples. High sensitivity protocol designed to work within an IgE concentration range between 1 and 20 ng/ml. Allows to analyze (serum) samples with IgE levels between 0.5 and 50 IU/ml.

IgEQUANT®- R

Receptor based sandwich ELISA Assay tool kit for quantitative determination of free human IgE in biological research samples. The kit utilizes recombinant human IgE high affinity receptor, a-chain (FceRIa), for coating of microtiter plate wells. Calibrated IgE standards and samples are added to the wells. Only free IgE, capable of binding to it's high affinity receptor, is measured. IgE neutralized by complex formation with therapeutic agents (e.g. omalizumab) or naturally occurring neutralizing anti-IgE auto-antibodies does not bind to the microtiter plate wells.

 

Each IgEQUANT® kit contains the capture antibody / recombinant IgE receptor, a biotinylated detection antibody, a calibrated reference standard, an avidin peroxidase conjugate and a detailed kit-specific protocol.

The IgEQUANT®-R kit utilizes a unique recombinant human FceRIa construct for IgE capturing, especially designed for maintenance of biologically active and sterically intact conformation after coating to microtiter plate wells.

 

IgEQUANT® kit quality control follows ICH and Clinical and Laboratory Standards Institute® guidelines. The IgE reference standard included in the kits is calibrated against the internationally accepted WHO IgE reference (WHO, 2nd IRP, 75/502).  Therefore, IgEQUANT® kits will deliver reliable results when used to establish ELISA assays for determination of total and free IgE serum levels in clinical research samples.

One IgEQUANT® research tool kit contains components required for the establishment of sandwich ELISA’s sufficient to run ten 96-well microtiter plates.